Comparing polydeoxyribonucleotide and polynucleotide for the treatment of ultraviolet b-induced photodamage and cutaneous inflammation in wistar rats

Authors

  • Jeanny Kusumawati Magister Program of Skin Aging and Aesthetics, Faculty of Medicine, Universitas Jenderal Achmad Yani, Cimahi, Indonesia.
  • Lili S P Meliala Magister Program of Skin Aging and Aesthetics, Faculty of Medicine, Universitas Jenderal Achmad Yani, Cimahi, Indonesia.
  • Rhea V Nugraha Department of Pharmacology, Faculty of Medicine, Universitas Jenderal Achmad Yani, Cimahi, Indonesia.
  • Khomaini Hasan Department of Public Health, Faculty of Health Technology, Universitas Jenderal Achmad Yani, Cimahi, Indonesia.

Keywords:

Inflammation, melanogenesis, PDRN, PN, UVB radiation

Abstract

Ultraviolet-B (UVB) radiation induces photodamage by activating pro-inflammatory pathways and degrading the extracellular matrix. This study aimed to compare the efficacy of polydeoxyribonucleotide (PDRN) and polynucleotide (PN) in modulating inflammatory status (IL-1β and IL-10), melanogenesis, and type I collagen expression in a UVB-induced Wistar rat model. Twenty-eight male Wistar rats were divided into four groups: a normal control group, an untreated UVB-exposed group, a UVB-exposed group treated with PDRN, and a UVB-exposed group treated with PN. Skin tissues were evaluated using Fontana–Masson staining and immunohistochemistry (IHC) to assess melanin, IL-1β, IL-10, and type I collagen, with quantitative analysis performed using ImageJ. Data were analyzed using one-way analysis of variance (ANOVA) followed by post hoc tests, with p < 0.05 considered statistically significant. Histopathological assessment revealed that the overall cellular and tissue morphology remained uniform, without apparent changes or distortion. However, quantitative analysis demonstrated distinct changes within these structurally preserved tissues. UVB exposure significantly increased IL-1β expression and melanin density (p < 0.001), while decreasing IL-10 and type I collagen levels. Both PDRN and PN reversed these effects, shifting the inflammatory profile toward resolution. By day 4, melanin density in both treatment groups had decreased to levels comparable to those of the normal control group. Concurrently, both treatments increased IL-10 expression and restored type I collagen levels, with the PN group demonstrating the highest collagen density. No statistically significant difference was observed between the PDRN and PN groups in terms of IL-10 modulation, indicating comparable anti-inflammatory effects. Overall, PDRN and PN effectively mitigated UVB-induced photodamage by restoring inflammatory homeostasis. This immunomodulatory effect was associated with reduced hyperpigmentation and enhanced collagen synthesis, highlighting the potential of both agents as regenerative therapies.

 

DOI : 10.35990/amhs.v5n1.p25-32

 

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Published

2026-06-30

How to Cite

Kusumawati, J. ., P Meliala, L. S. ., V Nugraha, R. ., & Hasan, K. . (2026). Comparing polydeoxyribonucleotide and polynucleotide for the treatment of ultraviolet b-induced photodamage and cutaneous inflammation in wistar rats. ACTA Medical Health Sciences, 5(1), 25–32. Retrieved from http://amhs.unjani.ac.id/index.php/acta_medica/article/view/130

Issue

Vol. 5 No. 1 (2026): ACTA Medical Health Sciences

Section

Articles